_bioid-reveals-overlapping-protein-interactome-for-cln3-6-8-and-new-roles-in-trafficking-of-synaptic-machinery.jpg "BioID reveals overlapping protein interactome for CLN3 6 8 and new roles in trafficking of synaptic machinery from 3 cln Video Screenshot Preview")
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⏲ Duration: 4 min 63 sec
✓ Published: 14-Oct-2020
✓ Published: 14-Oct-2020
Description:
Batten Disease (also known as Neuronal Ceroid Lipofuscinosis) is a family of rare genetic neurodegenerative disorders resulting from one of thirteen NCL genes. Cellular localization of NCL proteins has been well defined, but the function of many of these proteins remains largely undercharacterized. To address this problem, we expressed constructs consisting of a promiscuous biotin ligase fused to our proteins of interest (CLN3/6/8) in N2A cells to tag proximal proteins. N2A cells were lysed, bio
Batten Disease (also known as Neuronal Ceroid Lipofuscinosis) is a family of rare genetic neurodegenerative disorders resulting from one of thirteen NCL genes. Cellular localization of NCL proteins has been well defined, but the function of many of these proteins remains largely undercharacterized. To address this problem, we expressed constructs consisting of a promiscuous biotin ligase fused to our proteins of interest (CLN3/6/8) in N2A cells to tag proximal proteins. N2A cells were lysed, bio
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