_membrane-nanotube-pulling-assay.jpg "Membrane nanotube pulling assay from optical membrane Video Screenshot Preview")
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⏲ Duration: 13 sec
✓ Published: 20-Jun-2020
✓ Published: 20-Jun-2020
Description:
Giant unilamellar vesicles (GUVs) doped with biotynilated and fluorescent lipids are held in place by aspiration using a micropipette. A streptavidin-coated bead is then trapped using an optical tweezer and brought to proximity with the GUV in order to form a streptavidin-biotin bond. The bead is then pulled away from the GUV creating a membrane nanotube in-between the GUV and the bead.nnRendered using Blender2.8 + AE
Giant unilamellar vesicles (GUVs) doped with biotynilated and fluorescent lipids are held in place by aspiration using a micropipette. A streptavidin-coated bead is then trapped using an optical tweezer and brought to proximity with the GUV in order to form a streptavidin-biotin bond. The bead is then pulled away from the GUV creating a membrane nanotube in-between the GUV and the bead.nnRendered using Blender2.8 + AE
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