Is the Bystander Effect Real? from bystander effect cancer Watch Video

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Time-lapse imaging capturing transfer of eGFP from a NV1066 virus-infected JMN mesothelioma cell to a non-infected cell after de novo TNT formation. From Ady et al., Tunneling nanotubes: an alternate route for propagation of the bystander effect following oncolytic viral infection, published in Molecular Therapy Oncolytics December 7, 2016. nnDirect link to article: http://www.nature.com/articles/mto201629
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Additional time-lapse evidence of TNT formation between JMN (mesothelioma/cancer) cells infected or not infected with eGFP-expressing NV1066 virus (engineered form of oncolytic herpes simplex virus). Note the conformation of the TNT and its length >75 µm.nMovie S2 from Supplementary Information section of Ady et al., Tunneling nanotubes: an alternate route for propagation of the bystander effect following oncolytic viral infection, published in Molecular Therapy Oncolytics December 7, 2016. nnD
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MSTO-211H cells transfected with NV1066 form long-range connections via TNTs, which mediate intercellular transfer of virus-expressed eGFP. Cells were cultured in low serum, hyperglycemic medium for 48 hours.NV1066 HSV was added at MOI of 0.1.Time lapse images were captured every 10 seconds using a Zeiss LSM5 Live microscope.nPublished by Ady et al, Tunneling nanotubes: an alternate route for propagation of the bystander effect following oncolytic viral infection, Molecular Therapy-Oncolytic
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